Lepgen-96 Real-Time PCR System
1. Introduction
a. Stable and accurate heating and temperature control system
1. Adopt semi conductor thermo-electricity heating mode, the sample highest rise/reduce temperature speed able to achieve 5.0℃/s.
2. Adopt the semi conductor material with perfect thermal conductive performance, the temperature uniformity among holes achieved ±0.5℃
3. Accurate module temperature control mode guarantee quick and high accuracy temperature adjust and control
b. Perfect optics system
1. Adopt advanced long working life and high power LED light source with many advantages like energy saving, high efficiency, long working life, freely maintain, anti vibration, not easy to damage and quick response, etc
2. Applied reaction pipe side lighting technology, avoid affected by sample factors, improve fluorescence colletc efficiency and test sensitivity
3. Special light path design guarantee the uniformity of fluorescence test among each holes
4. Needn't ROC correction, no boundary effect
Excite eave length: wide excite wave length (300-800nm)≥4 passageways， can do best use more fluorescence signal
Test passageways: ≥4 passageways, guarantee at least test quadruple fluorescence at the same time, covered common probe and fluorescencecolourant in the market. And provide two customer made passageway for users, able to max realize sixfold fluorescence test.
c. Strong syn performance
Well situated flux: open type 96 holes flux design can guarantee daily requirements of lab, can suitable to most of the third party provided 8 units pipe and 96 holes plate in the market.
Dynamics range: wide range, can test 1-10^10 pieces copied DNA at the same time
Repetitiveness: high repetitiveness, CV<3%
Linear regression coefficient: r≥0.99
d.Strong software base system
1. Complete Chinese operating interface simple, clean and beautiful, guide type design more easy to operate
2. Projects in the software innovate design, visual and humanize
3. Strengthen department management, colourant management, experiment setting presave make information search, experiment manage and operation more easy
e. Complete analyse mode
Covered common experiment types: qualitative/absolute quantify, standard dissolve curve, the relate quantify, end point method equipotential gene parting and other
f. Flexible and convenient data analyse system
1. Sample multiply pipes mode design, realize multiply targets operating
2. Reaction progress, expansion increase curve and temperature trend can real time monitored, favour to user visualization observe the whole experiment process
3. Data analyse, data input and data output can self definition and flexible setting, can support input and output hospital system(HIS, LIS) data
Technical Parameters
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Model
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Lepgen-96
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Capacity
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96x 0.2ml tubes (low profile and standard) slides tube, 1x 96-well microplate
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Dynamic range
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1~1010 Copies
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Applied probe
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1st channel: FAM/ SYBR-Green
2nd channel: HEX, JOE, VIC, TET
3rd channel: ROX Texas -Red
4th channel: CY5
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Test method
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four-fold fluorescence detection
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Thermal cycle temperature control
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semiconductor thermoelectric module
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Temperature Range
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4.0°C - 99.0°C
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Heating and cooling Rate
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≥1.5°C/sec
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Thermal Accuracy
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≤0.5°C (at 90°C)
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Thermal Uniformity
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≤0.5°C (at 90°C)
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Hot cover temperature range
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50°C - 108°C
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Optical detection excitation wavelength
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300-800nm
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Detection wavelength
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500-800nm
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Fluorescence repeatability
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CV≤ 3%
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Fluorescent linearity
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r≥0.99 coefficient of correlation
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Software application
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genotyping by qualitative, absolute quantitative, standard solution curve, relative quantitative and endpoint method
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Scanning method
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full plate, specified rank scanning
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Data export format
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Excel, CVS
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Custom report form
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support
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The operating system
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Window 7/8/8.1/10
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Minimum computer configuration
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Intel or AMD Dual-2.8GHz, more than 4G memory and 128GB
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Power Supply
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AC 220V~, 50Hz, <800VA
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Communication Ports
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RS232, USB
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Size
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490mmX355mmX284mm
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Weight
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20Kg
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