Previously (April 19), the State Council's Medical Administration and Hospital Administration issued the "Notice on Further Doing a Good Job in New Coronavirus Testing During the Epidemic Period", requiring that all tertiary general hospitals should establish clinical tests that meet the biosafety level 2 and above standards. Laboratories with the ability to independently carry out new coronavirus testing. This time, the Health and Health Commission proposed that the testing institutions cover general hospitals above the second level, which means that the nucleic acid testing institutions will be expanded to counties, cities, and districts.
PCR laboratory applications:
1. It can greatly increase the trace amount of DNA. PCR is a conventional method for molecular biology research and experiments, and is widely used in various fields of biology.
2. Clinical testing: testing for AIDS, hepatitis B, avian diseases, oncogene testing and diagnosis, DNA fingerprinting, individual identification, and paternity testing.
3. Third-party testing: forensic evidence, animal and plant quarantine, testing of animals and their derived products, animal feed, cosmetics, food hygiene testing, genetically modified crops and genetically modified microorganisms, etc.
Clinical PCR Laboratory Workflow:
Reagent storage preparation→specimen preparation→DNA amplification→product analysis
1. Reagent preparation area:
The functions of this area are the preparation of storage reagents, the sub-packaging of reagents, and the preparation of the main reaction mixture.
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Balance
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MF1035C
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Used when formulating reagents and weighing materials
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PH meter
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OLABO-P10
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Used when measuring pH when preparing reagents
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Magnetic stirrer
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SH-4A
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Stir when preparing reagents (running buffers, stains, gels)
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-25°C refrigerator:
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BDF-25V350
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Reagents need to be stored at -20°C (required)
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Refrigerator
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BXC-250
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place reagents (required)
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Mixer
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VORTEX-1
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Mix the liquid in advance (required) before adding or preparing reagents
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Pure water machine
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OSJ-MT-10L
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For preparing pure water, preparing reagents and PCR system
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Pipette
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For pipetting liquids (required)
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2. Specimen preparation area:
The functions of this area are the preservation of clinical specimens, the extraction and storage of nucleic acids (RNA and DNA), and the addition of nucleic acids to amplification reaction tubes. In order to prevent the air in the adjacent area from entering this area, the pressure gradient should be set as positive pressure compared with the adjacent area.
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Safety cabinet
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BSC-4FA2
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The operation of adding biologically active samples (such as serum,whole blood, etc.) to the reagent plate needs to be carried out in a biological safety cabinet.
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Water bath
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Some extraction reagents may crystallize if stored at low temperature.
Before use, take a water bath to make the liquid clear. Some kits for manual nucleic acid extraction will have a water bath incubation step.
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PCR cabinet
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PCR-800
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The preparation of the PCR system needs to be completed in the PCR cabinet to prevent aerosol pollution.
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Microcentrifuge
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Mini-5
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Mix and centrifuge the sample before adding.
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Dry Thermostat
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BJPX-DB2
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Same function as water bath, can replace each other.
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Mixer
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VORTEX-1
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1. Mix the sample 2. After the PCR system is added, it should be fully mixed before putting it on the machine.
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Nucleic acid extractor
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BNP32
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Extract and purify nucleic acid.
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3. Product Amplification Region:
The role of this region is DNA amplification. The pollution index of the experimental amplification is relatively high, and the pressure gradient should be set to a negative pressure compared with the adjacent area. The advantage of this is that it can effectively prevent aerosol from leaking from this area.
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PCR instrument
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After the completion of the PCR instrument, agarose gel electrophoresis needs to be followed up, and the gel imaging system is used to observe after electrophoresis.
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Fluorescence quantitative PCR
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With the standard curve made by different concentrations of standards, the machine can directly give the original copy number of the sample, which can be absolutely quantified. This is the end of the experiment.
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4.Amplification product analysis area:
The role of this region is to detect relevant amplified fragments.
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Electrophoresis equipment
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After the PCR machine is completed, follow-up electrophoresis.
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Gel imaging system
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After agarose gel electrophoresis, observe the film with a gel imaging system to determine whether the target band is amplified.
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Listed above is a complete checklist for setting up a PCR laboratory.
As a manufacturer, OLABO is specialized in products of 8 areas, including medical diagnosis, biosafety protection, disinfection and sterilization, water purification system, cold chain products, software products, clean room project. OLABO can fully provide the relevant equipment used in the PCR chamber laboratory, and promises after-sales product training and installation guidance. We sincerely hope that more overseas customers can learn about these products, and it can be widely used in epidemic prevention and control around the world in the near future.
